Journal: Neuro-oncology

Article Title: Inhibition of nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme of the nicotinamide adenine dinucleotide (NAD) salvage pathway, to target glioma heterogeneity through mitochondrial oxidative stress.

doi: 10.1093/neuonc/noab175

Figure Lengend Snippet: Fig. 4 The effects of NAMPT inhibition on mitochondrial function. (A) Western blot analysis of SirT1 expression in the GSC262, GSC811, and GSC5-22 cell lines. (B) Assessment of the oxygen consumption rate (OCR). An Agilent Seahorse assay was used to determine the effect of KPT- 9274 on the basal respiration, maximum respiration, and spare respiratory capacity of the GSC262, GSC811, and GSC5-22 cell lines and the effect of adding 500 µM NMN, a pathway intermediate for the NAD salvage pathway, to reverse the effect of KPT-9274. (C) Immunofluorescence assays for mitochondrial morphologies, numbers, and membrane potentials in GSC262, GSC811, and GSC5-22 cells exposed to KPT-9274 or the vehicle and probed with 4′,6-diamidino-2-phenylindole (DAPI), Tom20-Alexa488, and MitoTracker Red CMXRos. Scale bar = 15 µm (D) Flow cytometric assessments of the mitochondrial potential of the GSC262, GSC811, and GSC5-22 cell lines exposed to 0.1 or 1.0 µM KPT-9274 or the vehicle and measured by staining with 2 µM JC-1 dye.

Article Snippet: After overnight incubation, cells were exposed to vehicle or 0.5 μM of KPT-9274 for 72 h. After incubation with MitoTracker Red CMXRos (20 nM) (Cat# 9082; Cell Signaling Technologies) for 30 min, cells were fixed with 4% formalin, permeabilized with 1% Triton X, and stained with anti-Tom20 (Cat# 42406; Cell Signaling Technologies) followed by Alexa Fluor D ow nloaded from https://academ ic.oup.com /neuro-oncology/article/24/2/229/6321319 by guest on 27 April 2024 233Sharma et al. NAMPT inhibition and mechanisms in gliomas N eu ro- O n colog y 488-conjugated anti-rabbit F(ab’)2 fragment (Cat# 4412; Cell Signaling Technologies).

Techniques: Inhibition, Western Blot, Expressing, Immunofluorescence, Membrane, Staining

Journal: BMC Complementary Medicine and Therapies

Article Title: Structure based docking and biological evaluation towards exploring potential anti-cancerous and apoptotic activity of 6-Gingerol against human prostate carcinoma cells

doi: 10.1186/s12906-023-04269-1

Figure Lengend Snippet: Intracellular ROS accumulation in PC-3 cells with DCFH-DA staining after 6-G treatment. A Photomicrograph showed ROS production intracellularly induced by 80, 100 and 120 µM of 6-Gingerol. Pictures were analysed phase contrast microscope. B Data are expressed numerically as fluorescence intensity percentage compared to control of PC-3 cells. Means ± SEM values are expressed of minimum three experiments independently, ** P < 0.01, *** p <0.001 and **** p <0.0001 in comparison to their particular control

Article Snippet: Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye, 4′,6-diamidino-2-phenylindole (DAPI), 2′,7′-Dichlorofluorescein diacetate (DCFH-DA), MitoTracker Red CMXRos, Acridine Orange (AO) and Propidium Iodide (PI) were purchased from Himedia, India for anticancer activity assessment.

Techniques: Staining, Microscopy, Fluorescence, Control, Comparison

Journal: Poultry Science

Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis

doi: 10.1016/j.psj.2026.106765

Figure Lengend Snippet: NRF2 inhibition results in impaired mitochondrial structure and function in Chicken PGCs. GSEA of genes related to mitophagy (A), citrate cycle (B), and oxidative phosphorylation (C) in ML385-PGCs versus Ctrl-PGCs. (D) TEM images showing subcellular structures of ML385-PGCs and Ctrl-PGCs. Yellow arrowheads indicate mitochondria with intact cristae; red arrowheads indicate mitochondria with disrupted cristae and vacuolization. Yellow arrows denote condensed chromatin in the nucleus. Scale bar: 2 μm. Original magnification, × 3. (E) Quantification of mitochondrial number per cell in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (F) Quantification of the percentage of vacuolated mitochondria in Ctrl-PGCs and ML385-PGCs based on TEM images ( n = 3 per group). (G and H) Assessment of mitochondrial activity using MitoTracker® Red CMXRos staining in ML385-PGCs and Ctrl-PGCs ( n = 3 per group). Scale bar: 50 μm. Statistical significance is indicated by * ( p < 0.05) and *** ( p < 0.001).

Article Snippet: Mitochondrial activity was assessed using the MitoTracker® Red CMXRos Kit (40741ES50, Yeasen, Shanghai, China) according to the manufacturer's instructions.

Techniques: Inhibition, Phospho-proteomics, Activity Assay, Staining

Journal: bioRxiv

Article Title: AtGPP2 encodes a 3-deoxy- manno- octulosonate-8-phosphatase required for the synthesis of KDO in rhamnogalacturonan II

doi: 10.64898/2026.05.02.722439

Figure Lengend Snippet: Subcellular localization of GPP proteins. Confocal laser scanning microscopy images of Arabidopsis cells expressing GFP, GPP1–GFP, or GPP2–GFP. GFP fluorescence, MitoTracker staining, chlorophyll autofluorescence, and merged images are shown. (A) GPP2–GFP or GFP alone. (B) GPP1–GFP or GFP alone.

Article Snippet: Seedlings were stained with MitoTracker Red CMXRos (Cambrex Bio Science Walkersville) and examined for GFP or MitoTracker fluorescence using a confocal laser scanning microscope (FluoView FV500, Olympus).

Techniques: Confocal Laser Scanning Microscopy, Expressing, Fluorescence, Staining